Bacillus subtilis, biofilm and construction and application thereof

The invention provides bacillus subtilis, a biofilm and construction and application thereof. A biofilm exhibition platform is a modified biofilm of the bacillus subtilis, wherein a TasA protein in the bacillus subtilis biofilm is modified into a TasA-R protein, is exhibited on the biofilm and has functionality. Gene modification and regulation and control of a main protein of the bacillus subtilis biofilm, namely a TasA starch sample protein, are carried out, and the exhibition platform of the bacillus subtilis biofilm is realized; compared with previous reports, the platform has the advantages of higher secretion capability, more complete functionality, higher biological safety, capability of being regulated and controlled and the like; the exhibition technology of the bacillus subtilis biofilm is widely applied.