Evidence of glycosylated sites on the endothelin-1 receptor in Swiss 3T3 cells

The effects of incubation of intact cells with six different lectins on the specific binding of [ 125 I]endothelin-1 (ET-1) were determined in Swiss 3T3 fibroblasts. ET-1 binding was unaffected by pretreatment of cells for 1 h at 37 o C with concanavalin A, soybean agglutinin, Ulex europaeus agglutinin 1, peanut agglutinin, or Galanthus nivalis agglutinin. However, preincubation of cells with 300 μg/ml of wheat germ agglutinin resulted in a 70% decrease in specific binding of ET-1 to cell-surface receptors