Supramolekulare Erkennungsstrukturen auf cellulosebasis

Starting from a novel type of amino cellulose derivative (so-called diamine celluloses) with an aromatic or aliphatic diamine residue, e.g. 1,4-phenylenediamine (PDA), at C-6 and with solubilizer groups such as tosylate, acetate, benzoate or carbanilate at C-2 or C-3, a supramolecular PDA cellulose derivative architecture can be synthesized, for example, with an oxidoreductase enzyme as the analyte recognition structure for biochemical sensor development. The structure consisting of glass/SiO 2 , PDA cellulose derivative and oxidoreductase can be achieved by the covalent coupling of the structural elements by means of bifunctionally reacting compounds, such as diketo compounds, in four reaction steps. The starting point is a transparent and ultrathin PDA cellulose derivative film on a chemically activated glass surface. The atomic force microscopy (AFM) image of the film shows a characteristic trough structure on a nanoscale, which points to a derivative-inherent supramolecular structuring (type I architecture). Glucose oxidase enzyme (GOD) can be incorporated into the PDA cellulose derivative architecture (type I) with high long-term stability (type II architecture, with recognition structure). The glucose affinity of the immobilized GOD is higher by a factor of 27 than in the case of the dissolved GOD enzyme.