RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF ETIZOLAM AND PROPRANOLOL IN PURE AND ITS TABLET DOSAGE FORM

A new reverse phase high performance liquid chromatography method has been developed for the simultaneous estimation of Etizolam and propranolol from their tablet formulation . The developed method was carried out on a C18(250x4.6mm) 5.0 µm column using a mobile phase of 0.02M sodium phosphate w/v : acetonitrile. (60:40) pH 6.5 with triethylamine. The flow rate was 1.0 ml/min with detection at 240 nm. The retention time for Etizolam 3.183 mins and for propranolol hydrochloride 4.863 mins.Etizolam and propranolol showed a linear response in the concentration range 4-50µg/mL, 4.5-50µg/mL respectively. The result of analysis have been validated statiscally and by recovery studies. The mean recoveries foµnd for Etizolam was 99.98% and for propranolol was 100.09%. The developed method was found to be simple, accurate, precise and selective for simultaneous estimation of Etizolam and propranolol Hcl in tablets.