Transformation of Kluyveromyces lactis

Often the aim of a transformation is to obtain at least one transformant that can be used in further experiments. This transformant should contain either a linear fragment integrated at the correct position in the genome or a plasmid. On the other hand, for transformation with a plasmid library, for example, high transformation efficiency is required. Therefore, the proce-dures for transformation of K. lactis described here are optimized to obtain as many transformants as possible.