Recherche de marqueurs diagnostiques précoces de l'adénocarcinome du pancréas par l'étude du transcriptome et du protéome

Pancreatic cancer is the 4th leadind cause of cancer death in human. To improve the poor prognosis of pancreatic cancer, new diagnosic markers need to be discovered in order to detect small pancreatic lesions. We used serial analysis of gene expression (SAGE) to identify 3 overexpressed genes that could potentially be used as cancer markers : PSCA (Prostate Stem Cell Antigen), S100A4 protein and mesothelin. Using a proteomic SELDI (Surface Enhanced Laser Desorption Ionization) - based technology on pancreatic juice samples, we identified HIP/PAP-I (Hepatocarcinoma Intestine Pancreas/Pancreatitis-Associated-Protein I) as an overexpressed protein in cancer samples.