Utilidad de la FISH en espermatozoides como análisis complementario de rutina en un laboratorio de andrología

Usefulness of FISH in sperm as a complementary routine analysis in the andrology laboratory Introduction: Fluorescent in situ hybridization (FISH) in decondensed sperm nuclei has proven to be a good method for determining the chro- mosome content of spermatozoa. Even among infertile men with a normal karyotype, there is a considerable frequency of chromosomal abnor- malities limited to the germ line. In the present study, we have retrospectively analysed our FISH results on spermatozoa in a series of patients with normal karyotypes who have a risk of sperm chromosomal abnormalities due to several factors such as, recurrent spontane- ous miscarriages, repeated implantation failures after ICSI and male factor. The objectives of the study were: a) to investigate whether these indi- cations were actually associated with an increased incidence of sperm aneuploidy and diploidy; b) to examine the correlation between sperm chromo- somal abnormalities on ICSI outcome in terms of fertilization, cleavage and embryo quality; and c) to find out if sperm FISH analysis could be used as a routine test in the andrology laboratory. Material and methods: Sperm aneuploidy and diploidy rates for chromosomes 13, 14, 15, 18,