[Simultaneous determination of six components in hair dyes by ultra performance liquid chromatography-tandem mass spectrometry].

A sensitive method was developed for the simultaneous determination of six components which included 4,4′-diaminodiphenylamine sulfate hydrate and 2,4-diaminophenol sulfate,etc. in hair dyes by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS / MS). After extracted by water through ultrasonic extraction,the samples were analyzed by UPLC-MS / MS. The separation was performed on a Waters BEH-C18column(100 mm × 2. 1 mm,1. 7 μm)with gradient elution of 10 mmol / L ammonium acetate and acetonitrile. The electrospray ionization(ESI)source in positive ion mode was used for the analysis of the six components in the multiple reaction monitoring(MRM)mode. The results showed good linear relationships with all the correlation coefficients(R2)more than0. 99. The limits of detection(LODs,S / N = 3)for the six components were in the range of 0. 26-4. 6mg / kg. The average recoveries of the six components in the spiked samples were in the range of 83. 0%-92. 2% with the relative standard deviations(RSDs,n = 6)of 5. 4%-11. 2%. The precision,accuracy,mean recoveries and the matrix effects satisfied the requirements of cosmetic sample measurement.The proposed method has been applied to the determination of six dyes in actual samples. This method is simple,accurate and effective for the simultaneous determination of the six components in hair dyes.