Direct high-performance liquid chromatographic separation of the racemates and diastereomers of nadolol

Abstract The direct separation of the two racemates and four diastereomers of nadolol by high-performance liquid chromatography (HPLC) is described. The racemates were well separated on non-chiral ODS columns using mixtures of phosphate buffer and methanol. The diastereomers could be separated on commercial chiral columns, using organic and aqueous mobile phases. The analytical HPLC method using an organic mobile phase was applied to semi-preparative HPLC for diastereomers.