Microheterogeneity of tubulin in the central nervous system of the quail (Coturnix coturnix japonica)

: Tubulin from the central nervous system of the Quail (Coturnix coturnix japonica) was analysed by two-dimensional gel electrophoresis. Either in soluble extracts or in tubulin enriched fractions, tubulin of forebrain, cerebellum and spinal cord is resolved into 3 components alpha, beta' and beta, characterized by their electrophoretic coordinates (alpha: pI = 5.4, MW = 54 kd, beta: pI = 5.2, MW = 52 kd; beta': pI = de mapping, by comparison with mouse brain alpha, beta and beta'-tubulin components. With highly resolutional isoelectric focusing 6 alpha isotubulins and 14 beta isotubulins are found in enriched-tubulin fractions of Quail forebrain and cerebellum.