Effect of Porophyllum ruderale (Jacq.) Cass. in the Liver of the B16-F10 Murine Melanoma Model and Antioxidant Potential

The aim of this study was to evaluate the Porophyllum ruderale effects on the liver in murine melanoma model B16-F10 and to determine the antioxidant potential. The plant’s aerial parts of the were collected in Campo Grande, MS, Brazil, dried, extracted with ethanol, ultrasonic bath and static maceration. The hydroalcoholic formulation at 100 and 200 mg/kg were submitted to antioxidant activity (DPPH) and hepatoprotective effect. Thirty-eight black mice with a mean weight of 30 g were distributed in 4 experimental groups: negative control, positive control (doxorubicin), P. ruderale at 100 mg/kg and P. ruderale at 200 mg/kg. All the animals received subcutaneous injection of 5x105 B16F10 cells on the 1st day of the experiment and were treated until the 14th day. Although a significant antioxidant activity was verified for the two concentrations analyzed, they were not sufficient to minimize the melanoma effects toxic in the mice liver as observed by histological changes and that leads us to conclude that the hydroalcoholic extract of aerial parts of P. ruderale, in the concentrations used,  possesses antioxidant properties, good performance in inhibiting the proliferation of human melanoma cells, but it has no hepatoprotective effect in the melanoma cutaneous treatment.    Keywords: Cutaneous Melanoma. Asteraceae. Hepatoprotective Effect. Phenolic Compounds. DPPH.   Resumo O objetivo deste estudo foi avaliar os efeitos de Porophyllum ruderale no figado em melanoma murino modelo B16-F10 e determinar o potencial antioxidante. As folhas da planta foram coletadas em Campo Grande, MS, Brasil, secas, extraidas com etanol, banho ultrassonico e maceracao estatica. A formulacao hidroalcoolica de 100 e 200 mg/kg foi submetida a atividade antioxidante (DPPH) e ao efeito hepatoprotetor. Trinta e oito camundongos pretos com peso medio de 30 g foram distribuidos em 4 grupos experimentais: controle negativo, controle positivo (doxorrubicina), P. ruderale na concentracao de 100 mg/kg e P. ruderale na concentracao de 200 mg/kg. Todos os animais receberam injecao subcutânea de 5x105 celulas B16F10 no 1o dia de experimento ate o 14o. Embora tenha sido verificada atividade antioxidante significativa para as duas concentracoes analisadas, elas nao foram suficientes para minimizar os efeitos toxicos do melanoma no figado dos camundongos, como observado nas alteracoes histologicas, o que nos leva a concluir que o extrato hidroalcoolico das folhas de P. ruderale, nas concentracoes utilizadas, possui propriedades antioxidantes, bom desempenho na inibicao da proliferacao de celulas de melanoma humano, mas nao apresenta efeito hepatoprotetor no tratamento do melanoma cutâneo.   Palavras-chave: Melanoma Cutâneo. Asteraceae. Efeito Hepatoprotetor. Compostos Fenolicos. DPPH.