14-3-3 protein of cerebro spinal fluid in diagnosis of creutzfeldt-Jakob disease

Objective To make detection of 14 3 3 protein in the cerebrospinal fluid of the patients with Creutzfeldt Jakob disease. Methods Western blotting was used to detect the 14 3 3 protein in the cerebrospinal fluid from 61 patients with Creutzfeldt Jakob disease and some other CNS disease. There were 5 patients with CJD, 18 patients with dementia were CNS diseases, 38 patients without dementia. The CSF samples from two patients with definite Creutzfeldt Jakob disease were used to test the stability of 14 3 3 protein. Both samples were stored under various conditions. Results The test was positive in all patients with Creutzfeldt Jakob disease and nagitive in all patients with dementia if the one patient with dementia who had had strokes within one month before testing was excluded. All the CSF samples tested that were storded under various conditions were clearly protein positive. Conclusion This should not be a screening test for patients without clinical dementia. It was suggested that the 14 3 3 immunoassay of the cerebrospinal fluid can be widely used to establish the premortal diagnosis of Creutzfeldt Jakob disease.