Protective protein for β-galactosidase, Ppgb, maps to the distal imprinting region of mouse chromosome 2 but is not imprinted

Abstract Human chromosome 20 is conserved as a single segment on distal mouse chromosome (Chr) 2. PPGB, protective protein for β-galactosidase, maps to human chromosome 20q13.1, and from linkage analysis of two interspecific crosses incorporating the mouse reciprocal translocations, T(2;8)2Wa (T2Wa) and T(2;16)28H (T28H), we have mapped the mouse homologue, Ppgb , to the conserved region on distal mouse Chr 2. From the combined data, the order of markers is T2Wa- Ada-Ppgb-D2Mit25 -T28H. Loci mapping between the T2Wa and T28H breakpoints lie in a region that is subject to parental imprinting, and so expression of Ppgb was tested in mice with maternal duplication/paternal deficiency and its reciprocal for the distal region of Chr 2. We have shown by reverse transcription-polymerase chain reaction (RT-PCR) that both parental alleles of Ppgb were expressed in the brain and kidney of 17.5-day-old embryos, 18.5-day-old embryos, and newborn mice. Thus, Ppgb does not appear to be subject to imprinting effects in the mouse.