Transplantatio no fLabeled Periodonta lLigamen tCell sPromotes Regeneratio no fAlveola rBone

Regeneratio no fdamage dperiodonta ltissue si smediate db yperiodonta lcells ,bu ta majo rsub-populatio ncomprise shighl ydifferentiate dcell stha td ono trenew .T oovercom eth elos so fspecialize dcel ltype scause db ydisease, variou stherapeuti capproache sincludin gcel ltransplant shav ebee ndevelope dt opromot ecel lre-populatio nin periodonta ltissues .A spreviou stransplantatio nstudie suse dunlabele dcells ,tha tar eindistinguishabl efro mhos tcells, i tha sbee ndifficul tt oasses sth econtribution so ftransplante dcell st oth ehealin gprocesses .T otrac kth efat eand differentiatio no fra tperiodonta lcell stransplante dint operiodonta lwounds ,w euse dcollagen-coate dfluorescen tbeads a sa permanen tendocytose dmarker ,o rcell sconstitutivel yexpressin gb-galactosidase .W eassesse dosteogeni ccell differentiatio nwit himmunohistochemica lstainin gfo rosteoponti nan dbon esialoprotein .Cell swer etransplante dinto periodonta lwound screate di nSprague‐Dawle ymal erat stha tar enul lfo rb-galactosidase .Defect swer eallowe dt oheal spontaneousl y(controls) ,o rwer eclose dwit hcollage nimplant smixe dwit hb-galactosidase-positiv e(Lac-Z )periodontal cells ,o rclose dwit hcollage nimplant smixe dwit hperiodonta lcell sloade dwit hfluorescen tbeads .Animal swer ekilled a t1 an d2 week safte rsurger yan dtissue swer eprepare dfo rmorphometri cassessmen tan dimmunostainin gfor osteoponti n(OPN )an dbon esialoprotei n(BSP) .Transplante dcell swer eeasil ydistinguishe db yfluorescen tbead so rby b-galactosidase-positiv eexpressio nan dwer edistribute dthroughou tth eregeneratin gperiodonta lligamen t(PL )and alveola rbone .A t1 wee kafte rwounding ,animal streate dwit hb-galactosidase-positiv ecell sexhibite da slightl yhigher percentag eo flabele dcell si nth eP Lcompare dwit hth efluorescen tbead-labele dcel limplan tgrou p(2 %vs .1% respectively ; P .0.2) .A tWee k2 percentage so flabele dcell swer eslightl yincrease di nth eregeneratin gP L(’3 %for bot hgroups , P .0.2) .I nregeneratin galveola rbon ea t1 week ,animal stha twer etreate dwit hb-galactosidase-positive cell san dfluorescen tbead-loade dcell sexhibite d’30 %an d25 %o flabele dcell srespectively .A t2 week safte rwounding ther ewa sa nincreas ei nth epercentag eo ftransplante db-galactosidase-positiv ecell s( ’39 %a twee k2 ; P ,0.05) ,but no to ftransplante dcell swit hfluorescen tbead s(’25 %a twee k2) .I nsite swit htransplante dcell sther ewer ehigher percentage so fOP Npositiv ean dBS Ppositiv ecell si nnascen tbon ean dmor enewl yforme dbon etha ni ncontrols (.40% ; P ,0.05) .Transplantatio no fb-galactosidase-positiv ecell so rcell sloade dwit hfluorescen tbead si sa useful metho dfo rassessin gth efat ean ddifferentiatio no fperiodonta lcell si nvivo .Fluorescen tbeads ,however ,ar edilute dat mitosi san dthi smetho dunderestimate sth epercentag eo ftransplante dcells .A stransplante dperiodonta lcell si nboth group spromote dregeneratio no falveola rbone ,cel ltransplantatio ncoul dimprov eth erestoratio no fperiodontium destroye db yperiodontitis .Ana tRe c262:193‐202 ,2001. ©200 1Wiley-Liss ,Inc. Ke ywords :periodonta lligamen tcells ;periodonta lwindo wwounds ;cel ltransplantation ;cell tracking