Phosphate dilution lowers net phosphatidyl choline synthesis in lymphocytes.

Abstract The rate of net phosphatidyl choline (PC) synthesis in lymphocytes was evaluated, under appropriate conditions,in terms of [ 32 P]phosphate incorporation. Phosphate-diluted and normal media were compared, with the cells being stimulated by concanavalin A or not. In either case, incubation in phosphate-diluted medium lowered net PC synthesis. In concanavalin A-stimulated cells, phosphate dilution also abolished the stimulation effect on net PC synthesis in G 1 . Nevertheless, these changes did not inhibit blastogenic transformation of cells.