[Study of rat's p53 gene damage and organ specificity induced by benzidine].

OBJECTIVE: Studying the main target organs and the genetic toxicity mechanism of benzidine. METHODS: SD rats were given benzidine i.p. injection. DNA was extracted from rat's liver, kidney, lung and bladders. Then ss probes RDPCR was used to detect the damaged DNA of exon 7 of p53 gene. RESULTS: Hybridization bands were found in liver, bladder and lung tissues after ss probes RDPCR, while no hybridization bands were found in kidney tissues. CONCLUSION: The result indicates that benzidine can cause the DNA lesions of exon 7 of p53 gene and its major target organs are liver, bladder and lung. The toxicity mechanism of benzidine is probably related to p53 gene damage.