Angiotensin II induction of fibronectin biosynthesis in cultured human mesangial cells: Association with CREB transcription factor activation☆

Abstract We assessed the effect of angiotensin II on fibronectin biosynthesis and transcription factor activation in adult human mesangial cells in culture. We found that 10 −5 mol/L angiotensin II tended to increase fibronectin mRNA expression within 1 hour (1.2-fold ± 0.3-fold of that in controls), with a significant increase after 4 hours (1.3-fold ± 0.1-fold of that in controls, p p 35 S)-labeled methionine into fibronectin after 2 hours (224% ± 23% of controls, p p 510 ) or without (FN 122 ) the CREB regulatory motifs. Angiotensin II resulted in a significant increase in CAT activity in FN 510 transfectants (1. 6-fold ± 0. 2-fold of that in controls, p 122 transfected cells. These data demonstrate that angiotensin II stimulates fibronectin biosynthesis in adult human mesangial cells and suggest that the process may be regulated at the transcriptional level.