Ovaryum dokusunun, vitrifikasyon ve yavaş soğutma teknikleriyle korunurluğunun histopatolojik yönden karşılaştırılması

Kadin hastalarda erken ovaryum yetmezligi ve infertilite kanser tedavisi yada baska nedenlerle ortaya cikabilir. Bu hastalarda fertiliteyi korumak icin ovosit, embriyo ve ovaryum dokusu saklanmasi yontemleri kullanilabilir. Ovaryum doku kriyoprezervasyonu, tedavisini geciktiremeyecek hastalar, prepubertal kiz cocuklari ve partneri olmayan kadinlarda uygulanabilir tek yontemdir ve iki teknikle uygulanmaktadir; yavas dondurma (slow freezing) ve hizli dondurma (vitrifikasyon). Gunumuzde, bu teknikler yeterli veri olmamasi nedeniyle standart bir tedavi secenegi olarak hastalara sunulamamaktadir.Bu calismada, fare ovaryum dokulari yavas dondurma ve hizli dondurma teknikleriyle kriyoprezerve edilerek saklandi. Cozulen dokulardaki hasarin isik, konfokal ve elektron mikroskobunda incelenerek kontrol grubuyla karsilastirilmasi ve histolojik yontemlerle degerlendirilmesi amaclandi.Uc grupta folikuller isik mikroskobunda sayilarak istatistiksel olarak birbirleriyle karsilastirildiginda; primordiyal ve primer folikullerin yavas dondurmada hizli dondurmaya gore daha cok korundugunu ortaya koyduk. Konfokal mikroskopta TUNEL boyama ile dokularda apopitozisin varligi gosterildi. Elektron mikroskobisinde dokularda normal folikullerde saglam ovosit cekirdegi, mitokondriyon basta olmak uzere membranli organellerin zarlarinda korunma, granuloza hucresinin baglantilarinda sureklilik, duzenli ZP gozlendi. Atretik folikullerde ise ovosit sitoplazmasinda sismis mitokondriyonlar, ZP'de kondansasyon, granuloza hucre baglantilarinda kopukluklar, granuloza hucre sitoplazmasinda bos alanlar, hucreler arasi alanda bos alanlar gozlendi.Sonuc olarak, yavas dondurma tekniginde hizli dondurmaya gore doku korunumunun daha fazla oldugu tespit edildi.AbstractCancer therapy and the other reasons can often induce premature ovarian failure and infertility. Oocyte, embryo and ovarian tissue freezing are developed options for fertility preservation. Ovarian tissue cryopreservation is essential in patients whoose care can?t be delayed, in prepubertal girls, women without a partner and have been applied in two techniques. There are two main ways of cryopreserving biologic tissue: either classic ?slow? freezing or vitrification by rapid cooling. Cause of insufficient researches, this way can not offer patient. Currently, these techniques haven?t been offered to patients since there hasn?t been enough data.In this study, mouse ovarium tissues have been preserved by vitrification and slow freezing. We examine tissue with light microscopy, electron microscopy and confocal microscopy to compare them with the control group and we aimed to evaluate them with histologic methods.We analyzed sections in light microscopy and counted the number of the follicles. Primordial and primary follicles have been preserved beter in slow freezing thecnique than in vitrification. Apopitosis is indicated in confocal microscopy with TUNEL method. Under the electron microscope, undamaged oocyte nucleus and organelles, stable junctions of granulosa cells, regular Zona pellusida have been observed in normal follicles. Swelled mitochondria in oocyte cytoplasm, condensation in ZP, breakage in junction of granulosa cell, space in cytoplasm of granulosa cell, vacuolization in extracellular space have been observed in atretic follicles.In conclusion, We found that slow freezing is more favorable than vitrification for ovarian tissue freezing.