Development of Validated Stability Indicating RP-HPLC Method for the Estimation of levo-Milnacipran Hydrochloride in Pure and Pharmaceutical Formulations

A simple, specific, accurate and stability indicating RP-HPLC method was developed and validated for the determination of levo-Milnacipran hydrochloride in pure and tablet dosage form. The chromatographic conditions comprised of a reverse-phase, Kromasil-(C18) column (250mm x 4.6mm, 5μ) with mobile phase consisting of a mixture of Methanol, Water and Acetonitrile in the ratio of 85:5:10(v/v). Flow rate (1.1ml/min). Wavelength (217nm), Retention time (6.7min) and the calibration curve was found linear between 30210µg/ml. The %recovery was found to be in the range of 99.22-100.22%. The method was validated and effectively separates the drug from its degradation product.