In Vitro Osteogenic Differentiation and Antibacterial Potentials of Chalcone Derivatives

Chalcone derivatives have been investigated for therapeutic agent such as anticancer, antioxidant and anti-inflammatory fields. In this study, we also have synthesized 4 different type of chalcone derivatives and demonstrated in vitro bioactivities. We divided into 2 groups of chalcones on the basis of similar substituents at aromatic rings and tested cell viability and proliferation potentials, indicating that methoxy substituent on A ring could enhance cytotoxicity and anti-proliferation potential depending on chalcone concentration. We also investigated osteogenic differentiation of C2C12 cells by ALP staining, which is demonstrated that the chalcones could not only induce activity of BMP-2, but also inhibit Noggin activity that is BMP antagonist. In addition, chalcone bearing hydroxyl groups at the 2-, 4-, and 6-position on the A ring inhibited growth of Streptococcus mutans, a major causative agent of dental caries. Therefore, we concluded that the chalcone derivatives synthesized in this research ca...