The antibody-based magnetic microparticle immunoassay using p-FET sensing platform for Alzheimer's disease pathogenic factor
2013
The importance of early Alzheimer’s disease (AD) detection has been recognized to diagnose people at high risk of AD.
The existence of intra/extracellular beta-amyloid (Aβ) of brain neurons has been regarded as the most archetypal
hallmark of AD. The existing computed-image-based neuroimaging tools have limitations on accurate quantification of
nanoscale Aβ peptides due to optical diffraction during imaging processes. Therefore, we propose a new method that is
capable of evaluating a small amount of Aβ peptides by using photo-sensitive field-effect transistor (p-FET) integrated
with magnetic force-based microbead collecting platform and selenium(Se) layer (thickness ~700 nm) as an optical filter.
This method demonstrates a facile approach for the analysis of Aβ quantification using magnetic force and magnetic
silica microparticles (diameter 0.2~0.3 μm). The microbead collecting platform mainly consists of the p-FET sensing
array and the magnet (diameter ~1 mm) which are placed beneath each sensing region of the p-FET, which enables the
assembly of the Aβ antibody conjugated microbeads, captures the Aβ peptides from samples, measures the photocurrents
generated by the Q-dot tagged with Aβ peptides, and consequently results in the effective Aβ quantification.
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