Synchronous detection of 3 kinds of quarantine viruses by multiplex RT-PCR method.

The specific primers were designed according to the conserved sequences of coat protein genes of three quarantine viruses-tomato ringspot virus(ToRSV),arabis mosaic virus(ArMV)and strawberry latent ringspot virus(SLRSV),and the single detection of ToRSV,ArMV and SLRSV was established by RT-PCR.Based on the above results a multiplex RT-PCR system of synchronously detecting those three viruses was preliminarily established by optimization of multiplex RT-PCR conditions.The experimental results showed that three pairs of the primers had strong specificity and could be used to amplify the 580 bp,438 bp and 298 bp target DNAs.This method was rapid,sensitive and accurate,providing a reference for synchronously detecting several kinds of viruses in introduced lily seedlings.