γδ T cells in the peripheral blood of individuals from an area of holoendemic Plasmodium falciparum transmission

Abstract γδ T cells bearing Vγ9 T cell receptors from unexposed Caucasian donors make large responses to Plasmodium falciparum in vitro . This finding, together with observations of others showing high levels of Vγ9 + T cells in the blood of infected non-immune individuals, led us to hypothesize that the response of these cells might contribute to the pathology of P. falciparum malaria. Acquisition of immunity to disease in people naturally exposed to infection may therefore be due in part to down-regulation or alteration of the function of γδ T cells. Supporting this view, and in contrast to infection in non-immune individuals, Vγ9 + T cells are not elevated in peripheral blood of children or adults living in an endemic area despite constant exposure to P. falciparum . After in vitro stimulation with P. falciparum , however, the expansion of Vγ9 + cells from the African donors is of similar magnitude to that observed for non-exposed Europeans. Thus, although these cells are not elevated in peripheral blood, they are still able to respond to P. falciparum antigens. In adult European donors the major γδ T cell population in peripheral blood is Vγ9 + (approximately 70% of all γδ cells), whereas in the majority of adult Africans Vγ1 + Vγ9 − T cells predominated (approximately 70% of total γδ cells).