Isolation and Identification of Anthocyanins from Morus nigra Linn.Fruits Grown in Xinjiang

This study dealt with the isolation of anthocyanins from Morus nigra Linn.fruits grown in Xinjiang by high-speed countercurrent chromatography(HSCCC).The solvent system used consisted of methyl tert-butyl ether,butanol,acetonitrile,water and trifluoroacetic acid(2:2:1:5:0.01,V/V).Fifty-gram sample was injected.As a result,4 individual anthocyanins with purities of 99.24%,88.5%,99.9% and 96%,respectively,were obtained.They were identified by HSCCC,UV-vis spectroscopy and LC-MS as cyanidin-3-O-rutinoside,pelargonidin-3-O-rutinoside,cyanidin-3-O-glucoside and pelargonidin-3-O-glucoside,respectively.The HSCCC method was efficient,stable,simple and easy.