Method for detecting N-acyl-homoserine lactone quorum sensing signal molecules in sample

The invention provides a method for detecting N-acyl-homoserine lactone quorum sensing signal molecules in a sample. The method comprises the following steps: (1) placing a pre-prepared semi-solid LB culture medium film on the upper surface of a TLC thin layer chromatoplate, wherein the semi-solid LB culture medium film contains report strains, and the sample is pre-outspreaded on the thin layer chromatoplate; (2) subjecting the film to a chromogenic reaction by utilizing X-Gal; and (3), confirming whether the sample contains the N-acyl-homoserine lactone quorum sensing signal molecules or not based on the results of the chromogenic reaction. The detection method provided by the embodiment of the invention has the advantages of obviously improved repeatability of experimental results, non-diffusion of chromogenic spots, clear color development, high resolution, low amount of X-gal, and high fidelity of detection results.