A software based application for cell proliferation determination

Abstract We evaluated a software based application for cell proliferation determination. Cells were cultured in different set-ups with and without cell-inhabitant agent. A scratch wound assay was performed to stimulate cell proliferation. The scratch wounds were photographed light-microscopically for 66.5 h. The images were software based evaluated in timely order by comparing each image to its predecessor using a Farneback algorithm. Compared to the manual evaluation, this application is timesaving, accurate, reproducible and of objective nature independent from the observer.