New approach for lsolating tumor cells and tumor Infiltrating lymphocytes

OBJECTIVE:To set up an efficient way to separate much more functional tumor infiltrating lymphocyte (TIL) from malignant pleura fluid and ascites. METHODS:TILs were isolated by two ways, i.e. the discontinuous density gradient centrifugation and the new attachment method established by authors.TILs were cultivated in the same conditions, and their proliferation as well as the killing activity and the phenotype changes were detected. RESULTS:The quantity of TILs was obviously higher by the new attachment method compared with that by the way of discontinuous density gradient centrifugation,and there were no differences in the proliferation or the killing activity in vitro as well as the phenotype changes between the two ways. CONCLUSION: The way of attachment is simple and efficient for cells separation,and it is deserved to be broadly applied.