Effect of Insecticides on Different Stages of Predatory Green Lacewing, Chrysoperla zastrowi sillemi (Esben.-Petersen)

Insecticides at their field recommended doses were evaluated for their safety against various developmental stages of Chrysoperla zastrowi sillemi. The levels of insecticide-detoxifying enzyme present in second instar larvae were measured using standard protocol. Carbosulfan @ 0.04 % caused the highest larval mortality, whereas pymetrozine was the least toxic. Among the treated larvae, pupation was the highest with thiamethoxam followed by buprofezin, cypermethrin and pymetrozine while adult emergence was maximum with azadirachtin. Among the insecticides, buprofezin was least toxic to the pupae of C. zastrowi sillemi. The synthetic pyrethroids, cypermethrin and bifenthrin, were highly toxic to all stages of C. zastrowi sillemi. The mean activity of cytochrome P450 in the second instar larvae of C. zastrowi sillemi was 23.0 mOD/chrysoperla/min and the estimated protein level was 0.7088–1.6300 nM/mg of protein. The mean acetyl choline esterase activity in test insect was 13.6 mOD/Chrysoperla/min and the protein content was 0.1442–0.7769 nM/mg. The mean activity of esterase in C. zastrowi sillemi was 64.6 mOD/Chrysoperla/min.