Effect of bio-fertilizers application on microbial diversity and physiological profiling of microorganisms in arable soil.

In laboratory assay, the diversity of bacteria and microscopic fungi and the community-level physiological profiling (CLPP) of microorganisms were observed after the addition of bio-sludge (40 t.ha -1 ) from a biogas station and addition of bio-fertlizers - AZOTER (10 dm -3 .ha -1 ) to the arable soil with PCR-DGGE and BIOLOG ® method (Eco Plates). The differences were recorded in the microbial diversity (bacteria and microscopic fungi) among variants according to the Shannon index. The differences in community of microscopic fungi were markedly higher among the soil samples with the additions of both  bio-fertilizers  compared to  control soil samples. The occurrence of individual OTUs (operational taxonomic units) bacteria and microscopic fungi were different after 105 days of incubation from the status after the 1 st day of incubation. The community metabolic diversity (CMD) was influenced by the incubation time (105 days) as well, but not by application of bio-fertilizers. We observed a significant decrease (LSD test, P <0.05) in community metabolic diversity (CMD) and average metabolic response  (AMR) of microorganisms in samples collected on the 105 th day of the experiment compared to  samples collected on the 1 st day of the experiment in all tested samples.