BBF RFC 45: Cloning Standard for Mammalian BioBrick Parts and Devices

The existence of multiple cloning standards presents a problem to biological engineers. Considering the weaknesses of the original BioBrick standard (does not allow for protein fusions), it is not surprising that a multitude of cloning standards has been proposed. As current discussion shows, each of the proposed alternatives has several disadvantages [1], but still, each standard is applied, for most registry parts are in the original BioBrick standard and compatibility is desired. RFC-12 is recognized as the most advanced standard with no disadvantages other than incompatibility with existing standards [1].