Qualitative and Quantitative Analysis of Bibenzyls from Dendrobium aurantiacum Rchb.f.var.denneanum

OBJECTIVE To establish qualitative and quantitative methods for the quality evaluation of Dendrobium aurantiacum var.denneanum.METHODS Two bibenzyls gigantol and moscatilin were identified by TLC.The separation of gigantol was obtained by RP-HPLC(Shim-pack CLC-ODS) using elution systems of acetonitrile-0.2% formic acid(65∶35) at 25 ℃.RESULTS Calibration curve showed good linear regression(r=0.999 9) within test ranges of 0.196~12.54 μg.Average recovery was 100.6% with RSD of 3.01%.CONCLUSION The developed methods were proved to be sensitive,reliable and useful by analysis of ten different samples from D.aurantiacum var.denneanum.The samples collected in April,May(Spring) and October,November(Autumn) accumulate much higher amount of gigantol than those of other months.