Genetic variation analysis of KAP3.1 gene in 6 rabbit populations.

Full-length CDS of KAP3.1 gene was first obtained by sequence assembly with 2 specific primers.Then the sequences mentioned above in 6 rabbit populations was scanned by PCR-SSCP.The results showed that 3 genotypes and 2 alleles were found at KAP3.1-1 loci,one SNP (C91T) which was silent mutation,while no mutation was found at KAP3.1-2 loci.The distribution of these genotypes in all populations was consistent with the Hardy-Weinberg equilibrium and showed low or no diversity;the distribution difference was significant or very significant (P 0.05 or P 0.01 ) from one to each other population except Fujian black rabbit and Jiuyishan rabbit,Fujian black rabbit and wan-line angora rabbit,chinchilla and white rex rabbit,Jiuyishan rabbit and white rex rabbit,jiuyishan rabbit and wan-line angora rabbit (P 0.05) .This study suggested a theoretical reference for whether KAP3.1 gene could be a molecular marker or not in the breeding of rabbit wool quality.