rDNA ITS Sequence Analysis of Different Germplasm of Salvia miltiorrhiza Bge

In order to study rDNA ITS sequence of Salvia,the rDNA ITS sequence of twenty-eight Salvia germplasm from different area were analyzed.The results showed that the length of rDNA ITS gene sequence of Salvia was 600~619 bp,including ITS1(227~228 bp),5.8S(166 bp) and ITS2(206~224 bp).There were thirty-one variable sites in the ITS1 area(it was 13.5% of total site),twenty-five variable sites in the ITS2 area(it was 11.2% of total site).The ITS1 and ITS2 gene fragments were conservative at specific level in Salvia,while there were less difference among some germplasm,so they could be molecular marker for authentication of Salvia.