Therapeutic ultrasound on bone cellular and in vivo adaptation

Objective: It is well documented that ultrasound, as a mechanical signal, can produce a wide variety of biological effects in vitro and in vivo. The purpose of the current study was to (1) develop a methodology to allow for in-vitro manipulating osteoblastic cells using acoustic radiation force generated by ultrasound, (2) use this methodology to determine the morphological and biological responses of bone cells to ultrasound, and (3) mitigate bone loss under estrogen deficient osteopenia. Methods: In Vitro Cellular Manipulation: We used a therapy focused transducer, which has spherical cap with 64 mm diameter and 62.64 mm focal length. A laser guide MC3T3-E1 osteoblastic cells were cultured in α-MEM containing 1% penicillin-streptomycin and 10% decomplemented newborn calf serum. In Vivo OVX Model: 72, 16 w.o. Sprague-Dawley rats were divided into six groups; baseline control, age-matched control, OVX control, OVX + 5 mW/cm2 ultrasound (US), OVX + 30 mW/cm2 US and OVX + 100 mW/cm2 US. Low intensity pulsed...