Modulating effects of β-naphthoflavone on the induction of SCEs by model compounds with emphasis on benzo[a]pyrene

1988 
Abstract The inducing capability of the synthetic flavonol β-naphthoflavone (β-NF) on cytochrome P-450 content was studied in primary chick embryo hepatocytes. In addition, the modulating effects of pretreatment with β-NF on the induction of siste-chromatid exchanges (SCEs) in V79 cells by mutagens from different chemical classes were investigated in a co-cultivation system consisting of primary chick embryo hepatocytes and V79 Chinese hamster cells. Finally, the effects of pretreatment on benzo[ a ]pyrene (B(a)P) metabolism were studied in more detail. Pretreatment of cultured primary chick embryo hepatocytes with β-NF resulted in a large increase in cytochrome P-450 content (a 2.8-fold increase after 31 h). Pretreatment with β-NF had no effect on the level of SCEs induced by N -nitroso-dimethylamine (NDMA) and 2-aminoanthracene (2AA). Pretreatment with β-NF resulted in a decrease in B(a)P-induced SCEs. This inhibitory potential was positively related to the β-NF dose. However, there was an inverse relationship between the inhibitory action of β-NF and the dose of B(a)P, at higher doses less inhibition was observed. When β-NF was applied simultaneously with B(a)P the percentage of decrease was about the same as for pretreatment. Pretreatment with β-NF followed by simultaneous application of β-NF and B(a)P did not result in larger effects.
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