Anti-HBV activities of Streblus asper and constituents of its roots.

Abstract The extracts from leaves, heartwood, barks and roots of the Streblus asper were investigated for anti-HBV activities, separately. The results suggested that the MeOH extracts of the heartwood, barks, and roots exhibited good anti-HBV activities. Further investigations displayed that ethyl acetate and n-butanol soluble parts of their MeOH extracts showed more significant anti-HBV activities. Moreover, a new lignan, together with 11 known compounds, was isolated from n-butanol-soluble part of MeOH extract of the roots of S. asper . The structures were elucidated by spectroscopic methods, including 1D NMR ( 1 H NMR, 13 C NMR), 2D NMR (HMQC, HMBC) and HR-EI-MS experiments. Compounds 1 – 3 were evaluated for their anti-HBV activities. Honokiol showed significant anti-HBV activity with IC 50 values of 3.14 μM and 4.74 μM for HBsAg and HBeAg with no cytotoxicity respectively, while lamivudine (3TC, positive control) exhibited weak anti-HBV activity with IC 50 values of 11.81 μM and 25.80 μM for HBsAg and HBeAg respectively.