Effects of Lactobacillus Probiotics on the Enhancement of Larval Survival of Portunus pelagicus (Linnaeus, 175) Fed Via Bioencapsulated in Live Feed

The aim of this study was to find out whether it is possible; the lactic acid bacteria (LAB) bioencapsulated with rotifers Brachionus plicatilis and Artemia franciscana can influence the survival of Portunus pelagicus larvae. Three indigenous LAB probiotics previously isolated from the gut of femal e Portunus pelagicus were used in the study. All LAB isolates were effectively grazed by B. plicatilis and A. franciscana when incubated at concentration s 10 cfu mL for 2 hours. Mixture of three LAB effectively 7 1 suppressed Vibrio in treated Artemia. LAB cfu per Artemia were decreased after inoculation in treated samples. No any LAB inhibited total growth of bacteria in Artemia. Survival of larvae was improved those fed with bioencapsulated live prey versus the untreated control. Enriched feed with Lactobacillus plantarum did produce highest survival 7.00 ± 0.87 %, followed by L. rhamnosus 6.83±1.26% survival. As an individual isolate, L. plantarum did show putative probiotic effect by producing better survival of larvae over other LAB probiotics. The survival counts of larvae were not statistically significant (P>0.05) in all cases. LAB was successfully recovered from the Artemia, rotifers, rearing water and larvae after post inoculation. The bioencapsulated live prey with LAB probiotics could be used as a tool for future studies on P. pelagicus.