Determination of curculigoside in crude medicine Curculigo orchioides by HPLC

Objective:To establish a method for the determination of curculigoside in Curculigo orchioides ,a species of crude medicine.Method:The methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C 18 cartridges to purify the solution,the curculigoside was detected by HPLC. Intersil ODS-3(150 mm×4.6 mm,5 μm) chromatographic column was used, mobile phase of methanol-water-ice acetic acid(45∶80∶1)and detect wavelength was set at UV 283 nm.Result:The average recovery was 99.2% for the detemination of curculigoside,RSD=1.7%( n =5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%.Conclusion:This method could be used to control the quality of crude medicine C. orchioides .