STUDY OF CLASTOGENIC POTENTIAL OF GLYCINE, INTERFERON-BETA, AND TRANSFER FACTOR, IN VIVO

Interferon-beta (IFN-B), transfer factor (TF), and glycine are agents that possess biomedical properties related to the modulation of the immune and the nervous systems, as well as to the inflammatory process. Therefore, safety evaluation of the compounds is necessary in regard to non genotoxic effect and safety use in human health. The present study evaluates their genotoxic and cytotoxic potential by determining the capacity of the compounds to induce sister chromatid exchanges (SCE), or to alter cellular proliferation kinetics (CPK) and the mitotic index (MI) in mouse bone marrow cells. Besides, it also determines their capacity to increase the rate of micronucleated polychromatic erythrocytes (MNPE) in peripheral mouse blood, and the relationship polychromatic erythrocytes /normochromatic erythrocytes (PE/NE) as an index of cytotoxicity. Two doses of each compound were tested. For the assays the mice received intraperitoneally (ip): 4x10 6 UI/ kg and 8x10 6 UI/kg of INF-B; 0.5 and 1 UI/kg of TF, 1 and 2 g/kg of glycine; 0.5 ml of distilled water (negative control group), and 5 mg/kg of cisplatin (positive control group) respectively. The results in regard to the agents showed no SCE increase induced by any of the tested doses, as well as no alteration in the CPK, or in the MI. With respect to the second assay, the results obtained with the tree agents were also negative for the MNPE and the PE/NE index along the daily evaluation made for four days. The results obtained establish that the studied agents have neither genotoxic nor cytotoxic effect on the model used.