Human CA125 protein quantum dot labeled double-sandwiched immunoassay detection kit

2014 
The invention provides a human CA125 protein quantum dot labeled double-sandwiched immunoassay detection kit. The human CA125 protein quantum dot labeled double-sandwiched immunoassay detection kit comprises the following components: CA125 protein standards with different fixed concentrations, a positive quality control product, a negative quality control product, 0.01M phosphate buffered solution with pH value of 7.2, polyclonal antibody diluent, a mouse anti-human CA125 monoclonal antibody coated ELISA plate, solution of CdTe quantum dot specifically combined with CA125 protein and mouse anti-human CA125 monoclonal antibody couplet, and 25*plate washing liquid. According to the kit, the polyclonal antibody used as a detection antibody, is labeled by the CdTe quantum dot, and the quantum dot labeling method has the characteristics of wide excitation spectrum, weak autofluorescence, stable fluorescence, long half-life period and the like, and therefore, the resolution ratio, the sensitivity and the specificity during detection can be greatly improved.
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