Protective role of aqueous medicinal herbal extracts against oxidative stress on Glucose-6-phosphate dehydrogenase activity and RBC fragility

In this paper we investigated the protective effect of the aqueous extracts of Salvia oficinalls, Thymus vulgaris and trigonella Foem-graecum against oxidative stress on Glucose-6-phosphate dehydrogenase (G6PD) activity and erythrocyte hemolysis. G6PD enzyme and red blood cells (RBC) were preincubated with aqueous extracts of different concentrations before adding nitric oxide (NO) donor compounds. The NO donor compounds nitrosocysteine and nitrosoarginine caused strong inhibition on G6PD activities. Those oxidative compounds promoted RBC hemolysis in parallel to their inhibition extents on G6PD activities. The complete protection of both G6PD enzyme activity and red blood cell hemolysis against oxidative stress induced by NO donor compounds was achieved by preincubation with the above mentioned aqueous extracts. Those extracts were able to protect hemolysis in parallel to their activation extents on G6PD activities. Compared to nitrosoarginine, nitrosocysteine showed more effect on both G6PD activity and red blood cells hemolysis. Thus, more aqueous extracts concentration is needed for protection against nitrosocysteine. (Wajdy Al-Awaida, Muhanad Akash. Protective role of aqueous medicinal herbal extracts against oxidative stress on Glucose-6-phosphate dehydrogenase activity and erythrocyte hemolysis. Life Sci J 2014;11(2):385- 391). (ISSN:1097-8135). http://www.lifesciencesite.com. 53 Keyword: Salvia oficinalls; Thymus vulgaris; trigonella Foem-graecum extracts; Glucose-6-phosphate dehydrogenase; Nitrosocysteine; Nitrosoarginine; aqueous extract.