O Klodronat Periferik Sinirlerde Yaralanma Sonrasi Rejenerasyon Sürecini Hizlandirir mi? Does The Clodronate Accelerate Regeneration Process After Peripheral Nerve Injury?

Aim: In this study, we aimed of the effects of LEC (LEC; Liposome Encapsulated Clodronate) an agent, to selectively deplete or reduce resident and systemic macrophages population during the degeneration-regeneration cycle after peripheral nerve injury. Materials-Methods: In our experiments, eight weeks old male wistar rats randomly divided into 5 groups as control (Group A), crush (Group B4 and B8) and crush+received LEC treatment (Groups C4 and C8) (n=40). Nerve crush injury model was performed on right sciatic nerve of the animals except the control group. Immediately after injury, LEC was intravenously injected after the days of postoperation 5, 10 and 15th (Group C). After 4-8 weeks following the injury, before EMG measurement was recorded than sciatic nerve dissected from Group B and C. Before and after 4-Aminophyridine (4-Ap) administration, the Compound Action Potential (CAP) were recorded with modified sucrose-gap system which is an in-vitro electrophysiological method from these branches Results: Our data show that LEC administration on damaged nerve did not significantly change CAP's parameters which are CV (CV; Conduction Velocity), amplitude of CAP, depolarization time (DT), latency and half width of CAP (p>0.05). However, LEC significantly changed the amplitude of delayed depolarization (del-dep) when occuring after administration of 4- Ap (p<0.05). Conclusion: The data obtained from this study, LEC may be reduce process of WD (Wallerian Degeneration) and accelerate functional recovery effect on remyelination after peripheral nerve injury.