Biological denitrification process based on the Fe(0)-carbon micro-electrolysis for simultaneous ammonia and nitrate removal from low organic carbon water under a microaerobic condition.

Abstract A combined process between micro-electrolysis and biological denitrification (MEBD) using iron scraps and an activated carbon-based micro-electrolysis carrier was developed for nitrogen removal under a microaerobic condition. The process provided NH 4 + –N and total nitrogen (TN) removal efficiencies of 92.6% and 95.3%, respectively, and TN removal rate of 0.373 ± 0.11 kg N/(m 3  d) at corresponding DO of 1.0 ± 0.1 mg/L and HRT of 3 h, and the optimal pH of 7.6–8.4. High-throughput sequencing analysis verified that dominant classes belonged to β -, α -, and γ - Proteobacteria , and Nitrospira . The dominant genera Hydrogenophaga and Sphaerotilus significantly increased during the operation, covering 13.2% and 6.1% in biofilms attached to the carrier in the middle of the reactor, respectively. Autotrophic denitrification contributed to >80% of the TN removal. The developed MEBD achieved efficient simultaneous nitrification and autotrophic denitrification, presenting significant potential for application in practical low organic carbon water treatment.