Intracellular Nanoparticle-Mediated Hyperthermia of Microscopic Tumours

Intracellular nanoparticle-based hyperthermia is said to be therapeutically superior to its extracellular counterpart for cancer treatment. [1] In nature biological system, primary cancer form a colony/cluster with before escaping into circulatory system to metastases. Therefore, evaluating the induced effects of intracellular hyperthermia treatment utilizing clusters of cancer would be more appropriate. Herein, simple experimental model evaluate the intracellular hyperthermia and its associated cytotoxicity using microscopic Jurkat tumorsphere. Paramagnetic nanoparticles (NPs) coating with anionic polymers are selected for the treatment (Fig. 1(a)). Cells were incubated nanoparticles at different concentrations. Prussian blue staining visualized the distribution of nanoparticles. Quantitative measurement of magnetic nanoparticles number was assessed by magnetophoresis. Cells were submitted to an alternative magnetic field (H=4 kA/m, f=21 kHz) and live/dead fluorescence assay analyzed the cytotoxicity effects at different exposure time. The particle number sufficient to induce cytotoxicity effects of Jurkat cell can be realized (Fig. 1(b)).