Immersion vaccination of Atlantic salmon ( Salmo salar ) against Yersinia ruckeri
2015
Yersinia ruckeri is a pathogen which causes yersiniosis and significant losses in farmed Atlantic
salmon ( Salmo salar ) in the Southern Hemisphere. Currently, Yersinivac-B, a commercial bacterin-based
vaccine manufactured by MSD Animal Health and prepared from formalin killed whole-cells,
is used for immersion vaccination against Y. ruckeri for most Tasmanian Atlantic salmon. Until
recently, these fish were vaccinated once by bath immersion at 5g. However, half a million
vaccinated juvenile Atlantic salmon died of yersiniosis in a single Tasmanian hatchery in 2007. In
this PhD study, we evaluated different inactivation methods of Y. ruckeri on the efficacy of single
dip vaccines including formalin inactivation, ammonium sulphate inactivation, and pH-lysed then
formalin inactivation. Additionally, hyperosmotic infiltration was combined with an ammonium
sulphate inactivated bacterin in immersion vaccination to assess the protection of these vaccines.
The relative percent survival (RPS) afforded was 93.4% for the fish vaccinated with ammonium
sulphate inactivated vaccine, 85.4% for fish vaccinated with formalin inactivated vaccine and
81.3% for fish vaccinated with pH-lysed then formalin inactivated vaccine. The results of this study
have shown that ammonium sulphate can be used to inactivate Y. ruckeri and can replace formalin
inactivation.
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