A new approch for structure determination of proteins by three-dimensional NMR.

Recent development of three-dimensional (3D) nuclear magnetic resonance (NMR) spectroscopy offers a new sight into the determination of the structures of larger proteins by increasing spectral resolution in three frequency axes. Simultaneously, problems of sensitivity loss due to larger linewidths associated with the increase of molecular weights has been solved by use of large three-dimensional NMR/protein structure/triple-resonance NMR/stable isotope