Intervensi Nikotin terhadap Level Low Density Lipoprotein, dan Ekspresi UCP-1 (Uncoupling Protein 1) pada Monyet Ekor Panjang (Macaca fascicularis) Obes dengan Resiko Aterogenesis
2010
Abstrak: Lima belas monyet ekor panjang obes (Macaca fascicularis) dengan resiko aterogenesis digunakan untuk memperoleh informasi tingkat low density lipoprotein (LDL) dan ekspresi protein uncoupling-1 (UCP-1) setelah tiga bulan intervensi nikotin dosis rendah per oral. Kelompok hewan dibagi menjadi tiga dengan komponen diet berbeda, yaitu diet A (lemak), diet tipe B (lemak dan kuning telur), dan diet tipe C (komersial diet-monyet chow), dan setiap kelompok terdiri dari lima monyet. Ketiga diet tersebut dicampur nikotin dan diberikan ke dalam makanan sehari dengan dosis 0,50-0,75 mg/kg/12 jam. Pengamatan terhadap lipid darah dilakukan setiap bulan dan pada saat nekropsi diambil jaringan adiposa coklat untuk evaluasi dengan teknik fluoresensi antibodi. Berdasarkan analisis lipid menunjukkan bahwa ada peningkatan yang signifikan konsentrasi LDL (p Abstract: Fifteen obese cynomolgus monkeys (Macaca fascicularis) were used to obtain information of LDL level and to study uncoupling protein-1 (UCP-1) expression during three months oral low dose nicotine intervention. Animals were grouped into three groups based on their diets, diet type A (tallow), type B (tallow and egg yolk) and type C (commercial diet-monkey chow), and each groups consisted of five monkeys. Nicotine was mixed into diet with dose 0.50-0.75 mg/kg/12 hours. Blood samples were colected every month for lipid analyses and during necropsy brown adipose tissues were taken for fluorescence antibody technique evaluation. Based on the lipid analysis showed that there was significant increase in LDL concentration (p Keyword: nicotine, expression UCP-1, Lipoprotein Low Density (LDL), cynomolgus
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