Chorionic gonadotropin beta subunit microfluidic disc and use method thereof

2016 
The invention discloses a chorionic gonadotropin beta subunit microfluidic disc and a use method thereof. The chorionic gonadotropin beta subunit microfluidic disc comprises a microfluidic disc body. A microfluidic detection unit is installed on the microfluidic disc body and comprises a whole blood injection groove, a whole blood separation channel, a blood cell storage groove, a plasma conveying channel, a mixing/detection groove, a magnetic particle solution injection groove, a cleaning fluid injection groove, an outer magnet area, a waste liquid tank, a human chorionic gonadotropin beta subunit monoclonal antibody solution injection groove and a light-emitting substrate solution injection groove, wherein the magnetic particle solution injection groove is wrapped by a human chorionic gonadotropin beta subunit monoclonal antibody, the outer magnet area contains a magnet, and the human chorionic gonadotropin beta subunit monoclonal antibody solution injection groove is labeled by alkaline phosphatase. By means of the simple method, intelligent control of microfluid in the microfluidic disc can be achieved. Meanwhile, reaction solutions can be fully mixed, it is guaranteed that a reaction system is efficient, and quantitative detection of the hCG concentration in samples is quickly achieved. The microfluidic disc has the advantages of being easy to operate, high in detection sensitivity, accurate and reliable in result, good in repeatability and low in cost.
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