Optimization of probiotic lactobacillus casei ATCC 334 production using date powder as carbon source
2012
This study was conducted to optimize culture conditions for economic
production of a probiotic bacterium, Lactobacillus casei ATCC 334, in which
palm date powder was applied for the first time as a low-cost main carbon
source. The effect of eleven factors on bacterial growth was investigated
using the Taguchi experimental design, and three factors including palm date
powder, tryptone and agitation rate were found to be the most significant
parameters. The optimum conditions including date powder concentration, 38
g/L; tryptone concentration, 30 g/L; and an agitation rate of 320 rpm were
determined by response surface methodology of Box-Behnken. A third-order
polynomial model was suggested to predict the design space following which
the predicted values were validated experimentally. The maximum log value of
the viable cells in the optimized alternative medium was 9.97 at 24 h of
incubation which was comparable to that obtained in the complex and expensive
MRS medium (10.06).
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
3
References
13
Citations
NaN
KQI