Raman spectroscopy based molecular bar coding: realizing the value of high wavenumber region in breast cancer detection

Here we present a ratiometric intensity analysis of the Raman spectrum in the HWN region of three breast tissue conditions, namely adipose, fibrous and malignant in a broad sampling of lumpectomy specimens. We demonstrate that these intensity ratios can be used as a “molecular barcode” in breast cancer pathology. Our ratiometric-based approach in HWN is simple, interpretable and exhibits distinct barcodes for different tissue types.